Table 1.
Primer pairs used in this study.
| Primers | Sequence (5′–3′) | Positiona | Product size (bp) |
|---|---|---|---|
| arcA | |||
| arcA-F1 | CTCAAGTCGGTGTCCATTCC | 17–36 | 280 |
| arcA-R1 | ACGTTATCCGGCGTGATCT | 278–296 | |
| arcA-F2 | AGATCACGCCGGATAACGTGGTACCGTGTTCAGCTTCTG | 838–857 | 317 |
| arcA-R2 | GTGTACGTGTTGCGGTCGTA | 1114–1133 | |
| arcA-F1 | CTCAAGTCGGTGTCCATTCC | 17–36 | 1117 |
| arcA-R2 | GTGTACGTGTTGCGGTCGTA | 1114–1133 | |
| arcA-comp-F | ACATGTCCCAAGCCATCCCTCA | (− 2)–20 | 1261 |
| arcA-comp-R | GCTCAGTAGTCGACGGGGTCGCG | 1237–1259 | |
| AF | CACAACCTGCTGACCGAGACCGTG | 217–240 | 220 |
| AR | CGCGAAACAGCGTGAGCACCTT | 415–437 | |
| arcC | |||
| arcC-F1 | GTATCGTCATCGCATTGGG | 5–23 | 277 |
| arcC-R1 | AGGTTGCCCATTTCCTGTTC | 262–281 | |
| arcC-F2 | GAACAGGAAATGGGGTACCTTGATCGACAAGGATCTGTGC | 608–627 | 334 |
| arcC-R2 | GAATCCCGTCGACCTTCAC | 901–916 | |
| arcC-F1 | GTATCGTCATCGCATTGGG | 5–23 | 915 |
| arcC-R2 | GAATCCCGTCGACCTTCAC | 901–916 | |
| arcC-comp-F | CCGCACCCCGGGCACCGTTGACACAA | (− 40)–(− 15) | 996 |
| arcC-comp-R | CGCGCGCCCGGGCGGCATCACCG | 934–956 | |
| CF | CGCGCAGACGGAAGGGATGAT | 228–248 | 240 |
| CR | CGGCACGACGCGGCGGAACTTG | 447–468 | |
| 16S | |||
| Univ_16S_F | TGGCTCAGAACGAACGCTGGCGGC | 21-44 | 336 |
| Univ_16S_R | CCCACTGCTGCCTCCCGTAGGAGGAGT | 327-356 | |
| pEXKm5 | |||
| OriT_F | TCCGCTGCATAACCCTGCTTC | 598-578 | 236 |
| OriT_R | CAGCCTCGCAGAGCAGGATTC | 368-383 | |
a
Positions corresponding to the nucleotide sequence of the indicated genes as annotated on B. pseudomallei K96243 chromosome 1 (NCBI Reference Sequence NC 006350) or the pEXKm5 sequence (GenBank accession number GQ200735).