Table 2.
Summary of altered-protein expression of stationary phase parental B. pseudomallei strain 153 type I and isogenic colony variant types II and III.
| Functional category | Altered protein | Spot number | Gene designation | Locus ID | Mass | PI | Score | Ratio type II/I or III/I |
Protein location/specific function | |
|---|---|---|---|---|---|---|---|---|---|---|
| II | III | |||||||||
| Upregulated proteins | ||||||||||
| Amino acid transport and metabolism | Arginine deiminase | 66 | arcA | BPSL1743 | 46,422 | 5.57 | 359 | 1.58 | 2.42 | Cytoplasmic |
| Arginine deiminase | 67 | arcA | BPSL1743 | 46,422 | 5.57 | 241 | 10.38 | 21.0 | Catalyzes the degradation of arginine to citruline and ammonia | |
| Carbamate kinase | 20 | arcC | BPSL1745 | 33,507 | 5.54 | 129 | 1.99 | 3.01 | Cytoplasmic | |
| Carbamate kinase | 45 | arcC | BPSL1745 | 33,507 | 5.54 | 130 | 6.67 | 10.13 | Reversible synthesis of carbamate and ATP from carbamoyl phosphate and ADP | |
| Hypothetical | 32 | – | BPSL1591 | 40,435 | 5.78 | 208 | 3.55 | 2.82 | Cytoplasmic | |
| Similar to Agrobacterium tumefaciens dehydrogenase | ||||||||||
| Energy production and conversion | Ferredoxin-NADP(H) reductase | 75 | fpr | BPSL0241 | 28,983 | 5.78 | 155 | 2.82 | 2.49 | Cytoplasmic |
| FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin | ||||||||||
| Carbohydrate metabolism | UDP-glucose dehydrogenase | 73 | udg | BPSL2511 | 50,802 | 5.34 | 93 | 1.53 | 3.47 | May have multiple localization sites |
| Cell wall/membrane biogenesis | ||||||||||
| Chaperone | Chaperonin GroEL | 29 | groEL | BPSL2697 | 57,137 | 5.13 | 159 | 0.51 | 3.00 | Cytoplasmic |
| 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions | ||||||||||
| Cell motility | Flagellin | 6 | fliC | BPSL3319 | 39,233 | 5.05 | 106 | 10.72 | 15.63 | Extracellular |
| Structural flagella protein | ||||||||||
| Down-regulated proteins | ||||||||||
| Lipid transport and metabolism | Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A | 53 | scoA | BPSL1955 | 25,373 | 5.6 | 155 | 0.103 | 0.105 | Cytoplasmic |
| Coenzyme A (CoA) transferases catalyze the reversible transfer of CoA from one carboxylic acid to another | ||||||||||
| Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B | 54 | scoB | BPSL1954 | 22,330 | 4.7 | 131 | 0.133 | 0.322 | Cytoplasmic | |
| Coenzyme A (CoA) transferases catalyze the reversible transfer of CoA from one carboxylic acid to another | ||||||||||
| Energy production and conversion | Inorganic pyrophosphatase | 22 | ppa | BPSL1021 | 19,206 | 5.37 | 88 | 0 | 0 | Cytoplasmic |
| Catalyzes the hydrolysis of pyrophosphate to phosphate | ||||||||||
| Betaine aldehyde dehydrogenase | 61 | aldA | BPSL1550 | 50,738 | 5.67 | 250 | 0.490 | 0.412 | Cytoplasmic | |
| Catalyses the conversion of betaine aldehyde to glycine betaine | ||||||||||
| Amino acid transport and metabolism/ | 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase | 74 | – | BPSL2169 | 29,660 | 5.68 | 181 | 0.136 | 0.386 | Cytoplasmic |
| Catalyzes the formation of N-succinyl-2-amino-6-ketopimelate from succinyl-CoA and tetrahydrodipicolinate in the lysine biosynthetic pathway | ||||||||||
| Inorganic ion transport and metabolism | Sulfurtransferase | 49 | sseA | BPSS1766 | 31,119 | 5.98 | 127 | 0.345 | 0.170 | Cytoplasmic Cyanide detoxification |
| Catalyzes thiosulfate and cyanide to sulfite and thiocyanate | ||||||||||
| Secondary metabolites biosynthesis, transport and catabolism | Non-ribosomally encoded peptide/polyketide synthase | 35 | phyH | BPSS1183 | 35,611 | 5.77 | 102 | 0.725 | 0.142 | Cytoplasmic membrane |
| Pseudomonas syringae syringomycin biosynthesis enzyme or B. thailandensis bactobolin | ||||||||||
| Posttranslational modification, protein turnover | Oxido-reductase | 62 | – | BPSL2748 | 23,904 | 5.75 | 95 | 0.085 | 0.081 | Cytoplasmic |
| Peroxidase | ||||||||||
| Antioxidant proteins | ||||||||||
| Multifunctional | Acetoacetyl-CoA reductase | 72 | phbB | BPSS1916 | 26,583 | 6.3 | 190 | 0.763 | 0.105 | Cytoplasmic |
| Synthesizes polyhydroxybutyrate (PHB) from acetyl coenzyme A (acetyl-CoA) in Ralstonia eutropha. | ||||||||||
| Unknown | Hypothetical protein | 71 | – | BPSL1549 | 23,384 | 5.14 | 66 | 0.417 | 0.091 | Unknown |
Protein spots were separated using a pH range 4–7 and examined using 2D Image master software. Only those proteins with a reproducible change in spot intensity volume between types I versus II or I versus III colony variants of ≥ 1.5-fold in two independent experiments are reported. Functional groups were identified based on COG functional category (http://www.ncbi.nlm.nih.gov). PSORTb was used to predict protein localization (http://www.psort.org/psortb).