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. Author manuscript; available in PMC: 2012 Jan 31.
Published in final edited form as: Insect Biochem Mol Biol. 2010 Aug 19;40(11):792–804. doi: 10.1016/j.ibmb.2010.07.007

Figure 8. An intrinsic fluorescent indicator of embryo development.

Figure 8

Blue auto-fluorescence can be detected in the yolk proteins. Change in the distribution pattern of blue fluorescence over 14 hours of development (stage 5-14, panel A) is seen in representative frames of time-lapse imaging. Blue fluorescence in the midgut is easily discernable in normally developed EPS-permeablized (1:10 EPS one minute) Stage 16/17 embryos (Panel B, B’). Permeabilized embryos exposed to 10 μM cycloheximide (CHX) shows gut malformation as seen by the blue fluorescence centrally localized in a “ball” of tissue or dispersed anteriorly and posteriorly (Panel C, C’. See text for discussion).