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. Author manuscript; available in PMC: 2012 Jan 31.
Published in final edited form as: Insect Biochem Mol Biol. 2010 Aug 19;40(11):792–804. doi: 10.1016/j.ibmb.2010.07.007

Figure 9. EPS treatment enables drug delivery.

Figure 9

2-3 hr embryos were treated (B) or not treated (A) with EPS (1:10, 30 seconds) and aged for an addition for 1.5 hours in PBS in a Kiehart chamber slide (see methods and Fig. 1F). Permeability was confirmed by the uptake of Rhodamine B dye (B’). Normal development to Stage 7-9 was scored by appropriate cephalic furrow (cf) formation and germband elongation (arrow) determined with DIC microscopy (A, B). Rates of normal and abnormal development resulting from combinations of exposures to EPS and CHX are tabulated (C). (n>180 embryos for each determination, expressed as percent +/- standard deviation).