Fig. 3.

Western blot analysis of expression of enzymes involved in the biosynthesis and catabolism of sulfatide. Expressions of CST, ASA, prosaposin, and Sap B in the cortices of PGC-1α KO and WT mice at 24 months of age were analyzed by Western blot analysis using GAPDH as a protein loading control. Four different cortices of WT and KO mice at 24 months of age were examined. The analysis revealed no change in expression levels of these proteins in the cortices of PGC-1α KO mice compared with those in WT mice.