Fig. 6.

Chronic long-term caloric restriction, not short-term caloric restriction, decreases apoE expression in mouse cortices. Expression levels of apoE in the cortices of WT and PGC-1α KO mice under AL or CR conditions at 5 months (A) and 24 months (B) of age were determined by Western blot analysis (see Materials and Methods). Expression of apoE was decreased by 40% under CR in both WT and KO mice at 24 months of age, but it was not changed at short-term CR, compared with WT and KO mice fed AL (C). Values represent the mean ratio of apoE expression levels in mouse cortices after normalization to the loading control GAPDH from four separate animals. Statistical significance was determined by a two-tailed Student t-test compared with that of WT mice under AL conditions; **P < 0.01.