TABLE 3.
Background PFAM levels from the solvents and plasticware
| PFAM | Background Amount for N18TG2 Cells | Background Amount for SCP Cells |
| pmoles per10 cells7 | pmoles per10 cells7 | |
| Palmitoleamide | 23 ± 7.0 | 140 ± 46 |
| Palmitamide | 12 ± 2.3 | 76 ± 15 |
| Oleamidea | 8.7 ± 4.1 | 55 ± 26 |
| Linoleamide | 14 ± 6.5 | 89 ± 41 |
| Tridecanamide | 17 ± 19 | 410 ± 450 |
To facilitate comparison of these data to the endogenous PFAM levels in the N18TG2 and SCP cells and to the cellular production data, the moles of amide found in these blanks was divided by an average number of viable cells (1.24 × 107 for SCP or 7.9 × 107 for N18TG2). The PFAM amounts are reported as the average ± SD for four determinations.
a
Each oleamide value actually represents the sum of the background levels of oleamide and elaidamide. Background levels of elaidamide (trans-9-octadecenic acid) are most likely ∼0 (3).