TABLE 4.
PFAMs in cells and media
| PFAM Origin | SCP Cells | SCP Media | N18TG2 Cells | N18TG2 Media |
| Tridecanoic acid | ||||
| 12 h | 2.0 ± 0.31 | 2.2 ± 0.92 | 0.10 ± 0.010 | 0.28 ± 0.23 |
| 24 h | 2.7 ± 0.75 | 3.2 ± 1.2 | 0.26 ± 0.12 | 0.50 ± 0.16 |
| 48 h | 2.3 ± 0.30 | 5.1 ± 0.43 | 0.75 ± 0.32 | 1.3 ± 0.37 |
| N-Tridecanoylethanolamine | ||||
| 12 h | 1.1 ± 0.28 | 2.0 ± 1.4 | 0.14 ± 0.068 | 0.26 ± 0.21 |
| 24 h | 2.7 ± 0.36 | 7.5 ± 1.6 | 0.28 ± 0.16 | 0.45 ± 0.037 |
| 48 h | 6.6 ± 1.5 | 10 ± 1.1 | 0.57 ± 0.27 | 1.0 ± 0.49 |
| Palmitoleic acid | ||||
| 12 h | 2.0 ± 0.36 | 3.8 ± 0.35 | 1.0 ± 0.77 | 1.9 ± 1.6 |
| 24 h | 3.1 ± 1.8 | 5.4 ± 2.1 | 5.8 | 8.5 |
| 48 h | 2.9 ± 1.6 | 7.9 ± 0.76 | 8.3 ± 1.3 | 15 |
| Palmitic acid | ||||
| 12 h | 3.7 ± 0.79 | 5.3 ± 1.4 | 6.2 ± 1.8 | 2.2 ± 1.0 |
| 24 h | 4.5 ± 1.6 | 6.4 ± 0.74 | 8.7 ± 3.7 | 21 ± 8.2 |
| 48 h | 33 ± 3.0 | 25 ± 8.4 | 21 ± 6.7 | 74 ± 27 |
| Oleic acid | ||||
| 12 h | 6.8 | 5.7 ± 3.7 | 0.82 ± 0.27 | 0.78 ± 0.32 |
| 24 h | 9.8 ± 2.9 | 17 ± 2.1 | 2.1 ± 0.71 | 1.4 ± 0.20 |
| 48 h | 10 ± 5.8 | 13 ± 3.9 | 2.1 ± 0.42 | 1.7 ± 0.13 |
| Elaidic acid | ||||
| 12 h | 12 ± 3.1 | 17 | 0.74 ± 0.20 | 1.7 ± 0.20 |
| 24 h | 21 | 51 ± 19 | 1.4 ± 0.32 | 1.9 ± 0.59 |
| 48 h | 21 ± 7.2 | 130 ± 19 | 6.7 ± 2.2 | 11 ± 0.74 |
| Linoleic acid | ||||
| 12 h | 4.0 ± 1.3 | 7.0 ± 1.7 | 0.35 ± 0.16 | 0.39 ± 0.044 |
| 24 h | 5.2 ± 0.73 | 26 ± 8.1 | 0.30 ± 0.12 | 0.41 ± 0.11 |
| 48 h | 29 ± 10 | 54 ± 30 | 1.3 ± 0.37 | 0.85 ± 0.27 |
Amounts are reported as nmoles/107 cells and are the average ± SD for two to three determinations. In a few instances, only a single determination was made. Blank values were subtracted from the amounts found in experimental samples prior to reporting: solvent blank for the cell samples and unconditioned media blank for the media samples. The PFAM amounts were measured after incubation of the indicated cells for 12, 24, or 48 h with media containing the corresponding free fatty acid.