Figure 10.

Renal uPA activity is increased after UUO and not influenced by Mrc2 expression until day 21. Renal uPA activity measured by plasminogen gel zymography showed similar levels on day 14 post UUO (A and B) and significantly higher levels in the Mrc2^−/− mice with worse fibrosis on day 21 (C and D). The uPA standard in the first lane on the left (Std) is purified low molecular mass human uPA (33 kD). The graphs (B and D) represent mean combined size of the two major uPA lytic bands (40–44 kD) measured using the Image Quant software program. The bars are mean ± SD. ^†P<0.05 for Mrc2^−/− versus Mrc2^+/+ groups.