Table 1.
Primers and conditions used to amplify the central repeat region of HRP2 and the polymorphic block 2 region of MSP1
| Primers | Primer sequences | Size (bp) | PCR conditions |
|---|---|---|---|
| HRP2 F | 5′-ATTCCGCATTTAATAATAACTTGTGTAGC-3′ | 74–968 | 95°C×2 minutes, 35 cycles of 94°C×30 seconds, 55°C×30 seconds, 72°C×60 seconds×35 cycles, and 72°C×10 minutes |
| HRP2 R | 5′-ATGGCGTAGGCAATGTGTGG-3′ | ||
| MSP1, conserved blocks 1 and 3 | |||
| MSP1 CONS F | 5′-ACTAGAAGCTTTAGAAGATGCAGTA-3′ | 522–639 | 95°C×2 minutes, 35 cycles of 94°C×30 seconds, 55°C×30 seconds, 72°C×60 seconds×35 cycles, and 72°C×10 minutes |
| MSP1 CONS R | 5′-AGTACGTCTAATTCATTTGCACGAA-3′ | ||
| MSP1, block 2 of K1 allotype parasites | |||
| MSP1K1 F | 5′-CTTAAATGAAGAAGAAATTACAAAAGGTGC-3′ | 140–266 | 95°C×2 minutes, 35 cycles of 94°C×30 seconds, 57°C×30 seconds, and 72°C×60 seconds |
| MSP1K1 R | 5′-GAGGGCTTGCACCAGATGAAGT-3′ | ||
| MSP1, block 2 of MAD20 allotype parasites | |||
| MSP1MAD F | 5′-GTATTAAATGAAGGAACAAGTGGAACAG-3′ | 157–193 | 95°C×2 minutes, 35 cycles of 94°C×30 seconds, 57°C×30 seconds, and 72°C×60 seconds |
| MSP1MAD R | 5′-TATCTGAAGGATTTGTACGTCTTGAATT-3′ | ||
| MSP1, block 2 of Ro33 allotype parasites | |||
| MSP1Ro33 F | 5′-AATAAAGGATGGAGCAAATACTCAAGTTGT-3′ | 153 | 95°C×2 minutes, 35 cycles of 94°C×30 seconds, 57°C×30 seconds, and 72°C×60 seconds |
| MSP1Ro33 R | 5′-TCTGAAGGATTTGCAGCACCTGGAGA-3′ | ||