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Analysis of binding model for the full-length Vpr-CypA interaction. SPR sensorgrams of the interaction of synthetic Vpr1-96 with immobilized CypA. Vpr1-96 was injected at concentrations 0-800 nM over CM5 chip immobilized with 180 RU CypA. The sensorgram was fitted to a 1:1 interaction model (A), heterogenous ligand kinetic model (B) and to a bivalent analyte kinetic model (C) using the biacore evolution program.
