Table 1. Relationship of IS2404 and ER-PCR results for Mycobacterium ulcerans and Buruli ulcer endemicity per village.

Numerical Village Designation	Village Name	Buruli ulcer Prevalence (cases/1000 pop)	#IS2404 positive/total sampled (%)	#ER positive/IS2404 positive (% pos)
1	So Ava	0	4/22 (18)	0/4 (0)
2	Vekky Degbadje	0	2/10 (20)	0/2 (0)
3	Tangnigbadji	0	1/9 (11)	0/1 (0)
4	Koundokpoe Center	0	2/11 (18)	1/2 (50)
5	Wedjame	0	0/8 (0)	0/0 (0)
6	Athieme	0	3/9 (33)	0/3 (0)
7	Zounhomne	0	7/8 (88)	1/7 (14)
8	Se	0	2/8 (25)	0/2 (0)
9	Guezin	0	4/8 (50)	0/4 (0)
10	Djebadji	0	5/8 (63)	0/5 (0)
11	Ahomey Lokpo	2.17	1/8 (13)	1/1 (100)
12	Vekky Daho	2.46	4/7 (57)	1/4 (25)
13	Ahomey-Hounmey	2.63	11/16 (69)	0/11 (0)
14	WoTogoudo	5.33	2/14 (14)	0/2 (0)
15	Djigbe Gbodje	6.06	2/10 (20)	2/2 (100)
16	Ahozonnoude	6.34	4/14 (29)	1/4 (25)
17	Zoungomey	6.75	7/13 (54)	1/7 (14)
18	Kento Augue	7.81	5/9 (56)	0/5 (0)
19	Akpome	10.6	8/16 (50)	6/8 (75)
20	Yamounto	20.27	12/15 (80)	6/12 (50)
21	Tchi-Ahomadegbe	29.04	5/10 (50)	2/5 (40)
22	Tandji	30.33	14/26 (54)	10/14 (71)

PCR was first used targeting IS2404. Those found to be IS2404 positive were further assayed using PCR targeting the enoyl reductase domain involved in mycolactone synthesis. Results represent the number positive from the total number assayed in a particular village.