Figure 4. The GABA_AR antagonist bicuculline prevents pentobarbital mediated inhibition of cortical and medullary neuronal activity.

Spontaneous activity of VRC (n = 45 neurons from 4 rats, filled circles) and cortical (n = 59 neurons from 4 rats, open squares) before bicuculline, and during bicuculline (blue line) and pentobarbital coapplication (blue and red lines). Individual units are normalized to the 30 min baseline preceding bicuculline treatment. The corresponding VRC neuronal activity during 300 µM pentobarbital application (Fig. 3) is overlaid for comparison (light green trace). * denotes different than pentobarbital treatment, p<0.05.