Figure 2.
Treatment of Tfhpx/hpx Hjv−/− mice. Tfhpx/hpx Hjv+/+ and Tfhpx/hpx Hjv−/− mice were treated at two months of age with 0.5 mL PBS, 10 mg transferrin or 0.5 mL washed wild-type RBCs every other day for one week then analyzed for (A) hemoglobin levels, (B) spleen masses, (C) serum iron levels, (D) total iron binding capacities, (E) liver iron levels, (F) total spleen iron levels and (G) liver Bmp6 and (H) hepcidin RNA levels relative to β-actin levels as measured by quantitative polymerase chain reaction. (A) ‘n’ refers to number of mice analyzed per group; each group contains male and female mice. Inset in (H) represents data replotted with a logarithmic y axis. Asterisks indicate statistical significance (P<0.05) relative to PBS-treated mice of the same genotype. ‘(wt)’ indicates Tf+/+ Hjv+/+ mouse values from Figure 1 included as historical reference. Bars on graphs represent one standard deviation.