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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Vaccine. 2011 Dec 14;30(6):1181–1190. doi: 10.1016/j.vaccine.2011.12.020

Figure 2. Flow cytometry analysis of CD28 and CD40L expression by CD4+ T cells following pneumococcal challenge.

Figure 2

Representative plots from three separate experiments are shown where spleen- and cervical lymph node (CLN)-derived CD4+ T cells from female F1 (C57BL/6 × BALB/c) mice, treated with control antibody (Ab, solid line) or anti-CCL5 Ab (dotted line) solutions, were isolated 7 and 14 days after intranasal challenge with Streptococcus pneumoniae strain EF3030. Mean fluorescence intensity (MFI) and fluorescence intensity histograms of CD28 and CD40L expression by CD4+ cells are illustrated and were analyzed using Flow Jo version 8.3 software. Underlined MFI values represent bacterial-challenged, anti-CCL5 antibody-treated groups.