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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Vaccine. 2011 Dec 14;30(6):1181–1190. doi: 10.1016/j.vaccine.2011.12.020

Figure 3. Flow cytometry analysis of CD80 and CD86 expression by CD11b+ and B220+ cells following pneumococcal challenge.

Figure 3

Representative plots from three separate experiments are shown where spleen- and cervical lymph node-derived CD4+ T cells from Female F1 (C57BL/6 × BALB/c) mice, treated with control or anti-CCL5 antibody (Ab) solutions, were isolated 7 and 14 days after intranasal challenge with Streptococcus pneumoniae strain EF3030. Mean fluorescence intensity (MFI) and fluoroscence intensity histograms of CD80 or CD86 expression by CD11b+ and B220+ cells are illustrated and were analyzed using Flow Jo version 8.3 software. Underlined values represent MFI recorded bacterial-challenged, anti-CCL5 antibody-treated groups.