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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Dec 2;1817(3):453–465. doi: 10.1016/j.bbabio.2011.11.011

Figure 4.

Figure 4

Time-courses of mitochondrial redox state (NADH) during states 2, 3 and 4 respiration comparing: effects of different substrates (Na+-pyruvate/malate (NaPM) (A,B) and Na+-succinate (NaSucc) (C, D)); effects of 0.5 mM added CaCl2 (≈200 nM free Ca2+) (A,C) vs. no added CaCl2 (B,D); and effects of isoflurane (1 mM) vs. rotenone (30 nM) vs. vehicle (DMSO). NADH redox state was normalized in each group as described in Fig. 3. Traces represent means of 4 replicates in each group. These data demonstrate similarities in the effects of isoflurane and rotenone (a known inhibitor of complex I activity), with different substrates and at different extra-matrix and matrix Ca2+ ([Ca2+]e, [Ca2+]m).