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. 2012 Jan 23;8(2):289–297. doi: 10.7150/ijbs.3520

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 3 — BIO promotes N2A cell-derived neurons back into tumor-like neuroblasts and up-regulates Id genes during this process. (A) Immunofluorescent staining of N2A cells and 2μM BIO treated N2A cells with anti-Nestin antibody (a and e, red), anti-Tuj1 antibody (c and g, red) and DAPI (b, d, f and h, blue). Scale bar= 200 μm. (B) Quantitative assessment of Nestin positive and Tuj1 positive cells in control and BIO-treated N2A cells. (C) Relative mRNA expression level of NSC marker Sox2 and Nestin in control and BIO-treated N2A cells. (D) Relative mRNA expression level of c-Myc and CyclinD1 in control and BIO-treated N2A cells. (E) Relative mRNA expression level of Ids in control and BIO-treated N2A cells.