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. 2011 Dec 29;13(1):383–392. doi: 10.3390/ijms13010383

Table 1.

List of the inter-simple sequence repeat (ISSR) primers used in the present study, polymorphism number, rates and polymorphism information content (PIC) values.

Primer name Nucleotide sequence Tm (°C) No. of fragments amplified No. of polymorphic fragments Percentage of poymorphism PIC value
ISSR-1 5′-(AC)8 G-3′ 54/9 23 19 82.6 0.313
ISSR-2 5′-(AC)8T-3′ 49/2 29 25 86.2 0.266
ISSR-3 5′-(AC)8C-3′ 53/3 25 20 80 0.291
ISSR-4 5′-(AG)8C-3′ 46/8 24 20 83.3 0.280
ISSR-5 5′-(AG)8T-3′ 42/5 28 26 92.8 0.205
ISSR-6 5′-(GA)8C-3′ 43/3 25 22 88 0.283
ISSR-7 5′-(GA)8T-3′ 42/9 36 26 72.6 0.333
ISSR-8 5′-(CT)8G-3′ 44/9 30 20 66.7 0.346
ISSR-11 5′-(AG)8GT-3′ 47/1 40 27 67.5 0.333
ISSR-14 5′-(AC)8GG-3′ 57 20 16 80 0.375
ISSR-15 5′-(AC)8GA-3′ 53/7 25 20 80 0.433
RAMP-TAG 5′-T(AG)9-3′ 56/1 29 24 82.7 0.428
RAMP-GAC 5′-G(AC)9-3′ 53/9 25 22 88 0.391
LK7 5′-CCA(CT)8-3′ 56/1 30 26 86.7 0.321
Total means - - 389 313 80.5 0.328