Table 1.
Primer name | Nucleotide sequence | Tm (°C) | No. of fragments amplified | No. of polymorphic fragments | Percentage of poymorphism | PIC value |
---|---|---|---|---|---|---|
ISSR-1 | 5′-(AC)8 G-3′ | 54/9 | 23 | 19 | 82.6 | 0.313 |
ISSR-2 | 5′-(AC)8T-3′ | 49/2 | 29 | 25 | 86.2 | 0.266 |
ISSR-3 | 5′-(AC)8C-3′ | 53/3 | 25 | 20 | 80 | 0.291 |
ISSR-4 | 5′-(AG)8C-3′ | 46/8 | 24 | 20 | 83.3 | 0.280 |
ISSR-5 | 5′-(AG)8T-3′ | 42/5 | 28 | 26 | 92.8 | 0.205 |
ISSR-6 | 5′-(GA)8C-3′ | 43/3 | 25 | 22 | 88 | 0.283 |
ISSR-7 | 5′-(GA)8T-3′ | 42/9 | 36 | 26 | 72.6 | 0.333 |
ISSR-8 | 5′-(CT)8G-3′ | 44/9 | 30 | 20 | 66.7 | 0.346 |
ISSR-11 | 5′-(AG)8GT-3′ | 47/1 | 40 | 27 | 67.5 | 0.333 |
ISSR-14 | 5′-(AC)8GG-3′ | 57 | 20 | 16 | 80 | 0.375 |
ISSR-15 | 5′-(AC)8GA-3′ | 53/7 | 25 | 20 | 80 | 0.433 |
RAMP-TAG | 5′-T(AG)9-3′ | 56/1 | 29 | 24 | 82.7 | 0.428 |
RAMP-GAC | 5′-G(AC)9-3′ | 53/9 | 25 | 22 | 88 | 0.391 |
LK7 | 5′-CCA(CT)8-3′ | 56/1 | 30 | 26 | 86.7 | 0.321 |
Total means | - | - | 389 | 313 | 80.5 | 0.328 |