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. 2012 Jan 4;13(1):506–515. doi: 10.3390/ijms13010506

Figure 3.

Figure 3

Overexpression of Tibetan porcine STING leads to upregulation of IFN-β and ISG15. IPEC-J2 cells were transfected with 2 μg/well of the plasmid pcDNA3.1(+)-STING or the empty vector pcDNA3.1(+). IFN-β and ISG15 mRNA levels were determined using real-time quantitative PCR 24 h after transfection. The amount of IFN-β and ISG15 mRNA was normalized to the amount of β-actin mRNA. Results are the mean and standard errors from three independent experiments performed in duplicate. * P < 0.05 and *** P < 0.001 as compared with empty vector.