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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Chem Res Toxicol. 2010 Jul 19;23(7):1286–1292. doi: 10.1021/tx1001755

Figure 1.

Figure 1

Western blot analysis for nitrotyrosine in proteins of APAP-treated hepatocytes. Freshly isolated mouse hepatocytes were incubated with APAP (1 mM) as described in Experimental Procedures. Controls were incubated with media alone. At 5 h, incubations were terminated, and 3-nitrotyrosine levels (protein nitration) were determined using Western blot analysis as described in Experimental Procedures. Each lane contains protein from a separate incubation performed on hepatocytes obtained from a separate mouse.