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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Chem Res Toxicol. 2010 Jul 19;23(7):1286–1292. doi: 10.1021/tx1001755

Figure 6.

Figure 6

Effect of NOS1 inhibitor on APAP-induced loss of mitochondrial membrane potential in freshly isolated hepatocytes. Hepatocytes were incubated with APAP (1 mM) or media alone for 2 h. Subsequently, hepatocytes were washed to remove APAP (arrow) and incubated in media alone (▼), media containing cyclosporine A (10 μM) (diamond), or media containing 7-nitroindazole (7-NI) (10 μM) (square). The control was incubated with media alone, washed for 2 h, and subsequently incubated with media alone (●). At the designated time mitochondrial membrane potential was determined using JC-1 as described in Experimental Procedures. *Significantly different from APAP treated at 2 h (hepatocytes from separate mice, n = 3, p < 0.05). †Significantly different from APAP-treated mice (hepatocytes from separate mice, n = 3, p ≤ 0.05).