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. Author manuscript; available in PMC: 2013 Jan 26.
Published in final edited form as: Neuron. 2012 Jan 26;73(2):317–332. doi: 10.1016/j.neuron.2011.10.038

Figure 1. GHSR1a and DRD2 mRNA and protein expression in mouse brain.

Figure 1

(a) GHSR1a and DRD2 mRNA expression in mouse striatum, hippocampus and hypothalamus. No PCR products are detected in controls containing no RT; the housekeeping gapdh was used as a negative control. The sizes of PCR products are: ghsr1a, 190 bp; drd2, 286 bp; and gapdh, 428 bp.

(b) Identification of neurons co-expressing GHSR1a and DRD2 in mouse brain. Brain sections from ghsr-IRES-tauGFP mice were stained with mouse DRD2 antibody and secondary Alexa647 (red); GHSR1a expressing neurons were localized by detecting GFP (green). To amplify GFP signal, sections were stained with GFP antibody and secondary Alexa488. Co-expression of GHSR1a and DRD2 is shown in overlay pictures with yellow arrows indicating individual neurons co-expressing GHSR1a and DRD2. Nuclei were stained with DAPI (blue). In striatum, box represents the area with higher magnification.

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