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. 2011 Dec 28;2:52–58.

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Published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.

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Figure 1. — Thin layer chromatography of P. reticulata extracts. Solvent system (v/v): acetone (10%), chloroform (75%) and formic acid (8%). Developer: NP/PEG. Extracts: hexane along (b), dichloromethane (d), ethyl acetate (f) and methanol (h). Standards: caffeic acid (a), tannic acid (c) (both are circled), chlorogenic acid (e) and rutin (g). Chromatography image (prepared on GF254 plate) is shown on the top right of the figures to indicate the caffeic and tannic acids in DM and EA extracts, under UV light and with no developer. Arrows indicate the phytochemicals, caffeic acid (green) and tannic acid (blue), in ethyl acetate extract. Note that dichloromethane extract shows a blue stain, suggestive of high tannin-content.