Skip to main content
. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Immunity. 2008 Dec 19;29(6):947–957. doi: 10.1016/j.immuni.2008.11.003

Figure 1. IL-22 is expressed in the inflamed colon.

Figure 1

(A) C57BL/6 CD4+ CD45RBhigh CD25− NK1.1− T cells (5×105) were transferred i.p. to RAG1−/− mice and five weeks post-transfer, when clinical signs of IBD were evident, cytokine mRNA in the colon was assessed by real-time RT-PCR. As a control, cytokine in the colons of RAG1−/− that did not receive T cells was also assessed. Bars represent the mean±SD expression of the cytokine gene to HPRT using the ΔΔCT method. Experiment was performed two times with similar results. 5–7 mice/group. ND=not detected.

(B) C57BL/6 CD4+ CD45RBhigh CD25− NK1.1− T cells (5×105) were transferred i.p. to RAG1−/− mice and at different weeks post-transfer (0, 1, 2, 3 and 4 weeks) cytokine mRNA expressed in the colon was semi-quantitated by real-time RT-PCR. Mean±SD; dashed line indicates limit of detection. 4 mice/group.

(C) From the colon samples in (A), a 1 cm section of the ascending colons of the above mice were cultured ex vivo for three days. Secreted IFNγ (left) or IL-22 (right) from the supernatants were quantiated by ELISA, mean±SD of 5–7 mice/group. ND=not detected, dashed line indicates limit of detection.