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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Dev Dyn. 2010 Apr;239(4):1178–1187. doi: 10.1002/dvdy.22277

Fig. 4. ETV2 directly activates transcription of a subset of endothelial and hematopoietic markers.

Fig. 4

Xenopus embryos were injected with mRNA encoding a dexamethasone (DEX) inducible version of ETV2 and cultured in the presence of DEX and/or the protein synthesis inhibitor, cycloheximide (CHX). Transcript levels for marker genes were quantitated for the various treatments using real-time qPCR. In all cases, transcript levels were normalized against transcripts of RNA polymerase 2. Graphs show results of triplicate biological replicates for each treatment. Presence of absence of DEX or CHX in a treatment is indicated by upper or lower-case type respectively. Statistical significance of differences between indicated samples is shown (P value measured by Student’s T test). The most important values are presented in the last two columns, which show transcript levels of putative target genes with uninduced ETV2 (−dex) and induced ETV2 (+DEX), both in the presence of CHX. The marker genes, flk1, aplnr, runx1 and spib appear to be direct targets of ETV2 regulation, whereas erg is probably not.