Table 2.
Inhibition of ETV2 function results in reduced expression of endothelial markers but not myeloid or erythroid markers
| Marker | Treatment | Normal gene expression % | Reduced gene expression % | Number of embryos |
|---|---|---|---|---|
| flk1 | etv2 control MO (50 ng) | 96 | 4 | 48 |
| etv2 control MO (25 ng) | 100 | 0 | 26 | |
| etv2 control MO (12.5 ng) | 100 | 0 | 21 | |
| etv2 MO1 (50 ng) | 34 | 66 | 29 | |
| etv2 MO1 (25 ng) | 23 | 77 | 40 | |
| etv2 MO1 (12.5 ng) | 24 | 76 | 82 | |
| etv2 MO2 (50 ng) | 19 | 81 | 29 | |
| aplnr | etv2 control MO (25 ng) | 96 | 4 | 46 |
| etv2 MO1 (25 ng) | 20 | 80 | 30 | |
| etv2 MO2 (50 ng) | 22 | 78 | 40 | |
| erg | etv2 control MO (25 ng) | 100 | 0 | 42 |
| etv2 MO1 (25 ng) | 8 | 92 | 25 | |
| etv2 MO1 (50 ng) | 0 | 100 | 29 | |
| etv2 MO2 (50 ng) | 11 | 89 | 37 | |
| runx1 | etv2 control MO (50 ng) | 100 | 0 | 44 |
| etv2 MO1 (50 ng) | 100 | 0 | 35 | |
| etv2 MO2 (50 ng) | 92 | 8 | 25 | |
| spib | etv2 control MO (50 ng) | 98 | 2 | 44 |
| etv2 MO1 (50 ng) | 97 | 3 | 32 | |
| etv2 MO2 (50 ng) | 100 | 0 | 22 | |
| α-globin | etv2 control MO (50 ng) | 94 | 6 | 17 |
| etv2 MO1 (50 ng) | 96 | 4 | 72 | |
| etv2 MO2 (50 ng) | 78 | 22 | 18 |