Fig. 1.

Schematic of the parts of the optical train in the Amnis flow cytometer that we used. The λ/2 waveplate and the linear polarizer were only inserted into the instrument when measurements were made with the polarization of the 785 nm light scattering laser perpendicular to the scattering plane. Camera 1 was used for the brightfield, lysosomal and mitochondrial images while camera 2 collected the nuclear images.