Fig. 2.

Example raw images: a) Brightfield b) Lysosomes c) Mitochondria d) Nuclei e) Side scatter (SSC). The yellow lines are the outline of the mask used for the cell. Other colored lines are the outlines of masks covering areas where the dye fluorescence is considered significant. Black lines are the outlines of regions where only the dye in that image fluoresces. The mask for the non-fluorescent regions is shown on the SSC image. The thick white line on the Hoechst image has a length of 10μm.