Skip to main content
. 2011 Dec;23(12):4173–4183. doi: 10.1105/tpc.111.093880

Table 1.

Characteristics of Group VII ERFs in Arabidopsis Related to the Hypoxic Response and Degradation by the N-End Rule Pathway

Gene Name HRE1* HRE2* RAP2.3 RAP2.2** RAP2.12**
ATG code At1g72360 At2g47520 At3g16770 At3g14230 At1g53910
Control expression level (root/shoot)a 187/103 45/93 408/4464 507/892 1114/574
Hypoxic expression level (root/shoot)a 7059/301 2647/1239 498/3051 554/950 750/403
Presence of MC at N terminus Yes Yes Yes Yes Yes
Protein degradation in vitrob Yes Yes Yes Yes Yes
Protein degradation in planta Yesb Yesb Not tested Not tested Not tested
Protein accumulation under hypoxia Nobb Yesb Not tested Not tested Yesc
Effect of lower gene expression Slightly lower induction of ADH activity in T-DNA insertion lined; lower induction of hypoxic genes in RNAie unchanged induction of ADH activity in T-DNA insertion lined; decreased salt tolerancef T-DNA insertion is less tolerant to hypoxia, lower ADH induction under hypoxiaj
Effect of overexpression More tolerant to hypoxiabg; higher ADH expressiondg More tolerant to hypoxiabg; unchanged gene expressiong; increased tolerance to floodingf; increased salt tolerancef Higher expression of defense genes (PDF1.2, GST6)i More tolerant to hypoxia, higher ADH induction under hypoxiaj; RAP2.2 protein does not accumulate, little gene expression changes under controlk More tolerant to submergence, higher induction of hypoxic genesc
Interacting partnersh At2g38490 (CIPK22, SnRK3.19) ACBP2l, ACBP4m, OBF4n, TTL3, RCD1 SINAT2 (Ring finger protein, At3g58040)k ACBP1c, ACBP2c, At2g44040
Other remarks mRNA induction inhibited by CHXg mRNA induction not inhibited by CHXg Involved in defense response?i Induced by darknessj; involved in regulation of carotenoid biosynthesisk
Intracellular localization Nucleus (N-terminal tag)c Nucleus (N-terminal tag)cf Nucleus (N-terminal tag)i; Nucleus and cytosolm Membrane (nucleus under hypoxia, C-terminal tag)c

*An HRE1/HRE2 double T-DNA insertion line was less tolerant to hypoxia and showed lower induction of hypoxic genes (late response; Licausi et al., 2010). ** RAP2.2/2.12 amiRNA-plants (knockdown for both genes) showed reduced induction of hypoxia-responsive genes (Licausi et al., 2011a). CHX, cycloheximide; MC, N-terminal Met-Cys.

a

Data are expression values (RMA-normalized) from roots and shoots of Arabidopsis seedlings (Mustroph et al., 2009).

h

Arabidopsis Interactome Mapping Consortium, 2011, http://interactome.dfci.harvard.edu/A_thaliana/.