Figure 8.

Differential Expression of Sequence Variants in the Same miRNA Family.

miRNA expression data from 2-week-old root, shoot, and 15-cm panicle were mean centered and represented by a heat map. The sequences of miRNA family members were aligned, and nucleotides that differ are shown in red. Validation of the expression patterns was accomplished by splinted-ligation-based miRNA detection. miR168a was used as constitutive expression control. miR156 family (A), miR171 family (B), miR164 family (C), miR166 family (D), and miR172 family (E).