Figure 9.

Subcellular Localization of GFP-Tagged RGH3 Proteins.

(A) Transient expression of GFP alone (top panels), RGH3α with a C-terminal GFP fusion (middle panels), and the RGH3^EMS allele with a C-terminal GFP fusion in Arabidopsis mesophyll protoplasts (bottom panels).

(B) Transient expression of the RGH3α-GFP fusion in Arabidopsis suspension cell culture protoplasts shows dual targeting in subnuclear compartments. Percentages are the frequency of GFP localization patterns from 200 nuclei in two biological replicates.

(C) Transient expression of N-terminal GFP fusion proteins in N. benthamiana. RGH3α is the full-length RGH3 protein. RGH3^umu1α is the full-length protein with the 47–amino acid indel polymorphism caused by the rgh3-umu1 allele. Additional RGH3 variants are truncated forms encoding the N-terminal acidic domain (ζ) or the acidic domain and one zinc finger (β, γ). U2AF⁶⁵a shows subnuclear localization to speckles in the absence of RGH3α.

(D) Transient coexpression of C-terminal fusions of RGH3α-GFP and U2AF⁶⁵a-RFP in N. benthamiana.

Bar = 5 μm in all panels.