Figure 3.

OSH1 and OSH15 Are Redundantly Required for SAM Formation and Maintenance during de Novo Shoot Regeneration from Callus.

(A) Regenerated (left) and germinated (right) seedlings of osh1. White arrowheads indicate the position of each leaf.

(B) and (C) Sections of shoot apices of the regenerated wild-type (B) and osh1 plants (C). Arrowheads indicate the SAM.

(D) Panicles of regenerated plants of the wild type (wt) (left) and osh1 (right).

(E) and (F) Spikelets formed on regenerated plants of the wild type (E) and osh1 (F). a, anther; p, pistil; asterisk, abnormal short anther of osh1.

(G) and (H) Regenerating shoot from the wild type (G) and osh1 d6 (H). Arrows indicate leaf-like structures formed from osh1 d6 callus.

Bars = 2 cm in (A), 50 μm in (B) and (C), 5 cm in (D), and 2 mm in (E) to (H).