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. 2011 Dec 29;23(12):4368–4381. doi: 10.1105/tpc.111.090050

Figure 4.

Figure 4.

Expression Levels of KNOX Genes Were Reduced in osh Mutants.

(A) Expression of KNOX genes in germinating embryos. Relative expression level was calculated as the ratio to the expression level at 2 DAG in the wild type (wt) for each gene.

(B) Expression of KNOX genes in the SAM formation stage of the embryo at 5 DAP. Relative expression level was calculated as the ratio to the expression level in the wild type for each gene.

(C) In situ hybridization of OSH6 and OSH71 in wild-type and osh1 d6 embryos at around the stage of SAM formation. Bars = 100 μm.

(D) Expression of KNOX genes in callus before and 1 week after CK treatment. Relative expression level was calculated as the ratio to the expression level before CK treatment in the wild type for each gene.

In (A) to (C), the expression level of ubiquitin (ubq) was used as an internal control for normalization. The data represent the average of three independent biological replicates. Error bars represent the sd. Asterisks indicate significant reduction at P < 0.05 compared with the expression level in the wild type.