Figure 7.

Positive Autoregulation of OSH1 Is Essential for Its Expression and SAM Maintenance.

(A) Reporter gene structures. Gray lines indicate introns and 5′ and 3′ regions. White and green boxes indicate exons and GFP, respectively. In the case of GFP-mOSH1, OSH1 binding sequences were substituted from TGAC to TCTC.

(B) GFP-OSH1 expression in the shoot apex.

(C) GFP-mOSH1 expression in the shoot apex.

(D) Regenerating shoots from osh1 d6 callus carrying GFP-OSH1 on regeneration medium.

(E) osh1 d6 callus carrying GFP-mOSH1 on regeneration medium. For regeneration, transgenic osh1 d6 calli were cultured on regeneration medium for 4 to 5 weeks.

(F) A rescued osh1 d6 plant carrying GFP-OSH1 grew until the reproductive phase.

(G) d6 and wild-type (wt) plants during the reproductive phase.

Bars = 100 μm in (B) and (C), 5 mm in (D) and (E), and 10 cm in (F) and (G).