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. 2011 Dec 30;23(12):4462–4475. doi: 10.1105/tpc.111.089680

Figure 2.

Figure 2.

Arabidopsis LTO1 Partially Complements the E. coli dsbAB Mutant for Disulfide Bond Formation.

Strains are wild-type HK295 (pBAD24), ΔdsbAB HK329 (pBAD24), and ΔdsbAB HK329 (pLTO200).

(A) Complementation of the motility phenotype in dsbAB mutants by Arabidopsis LTO1. Representative motility phenotype of wild-type (WT), ΔdsbAB, and ΔdsbAB bacterial strains expressing LTO1 under an Ara-inducible promoter (+/−ara) on M9 solid media.

(B) Quantification of motility phenotype represented in (A). Each bar represents the sd from 10 independent plate measurements.

(C) Complementation of the phoA-deficient phenotype in dsbAB mutants by LTO1. Each bar represents the sd from three separate measurements of PhoA activity. A.U., arbitrary units.

(D) Complementation of the growth phenotype on MacConkey agar in dsbAB mutants by LTO1. In all plates in (D), the top left sector was inoculated with HK295 (pBAD24), the top right sector was inoculated with HK329 (pBAD24), and the bottom sector was inoculated with HK329 (pLTO200).