Figure 5.

Accumulation of Cytochrome b₆f and PSI Is Not Affected by Loss of LTO1.

Wild-type (WT) and lto1 homozygous plants were analyzed for the accumulation of cytochrome b₆f subunits (cytochrome b₆, cytochrome f, PetC, and PetD), PC, and PSI subunit PsaA via immunoblotting. Heme staining was also performed to analyze the accumulation of holoforms of cytochrome b₆ and f. Total protein samples corresponding to 16 μg of chlorophyll were separated on a 12% SDS-acrylamide gel. For an estimation of the protein abundance in the lto1 mutant, dilutions of the wild-type sample were loaded on the gel. Gels were transferred to membranes before heme staining by chemiluminescence or immunodetection.