Table 1.

Topological Analysis of Arabidopsis LTO1 from PhoA and LacZ Fusion Analysis

Fusion	Position	LacZ	PhoA	Topology
1	74	73 (±22)	4 (±3)	n-side
2	113	19 (±4)	78 (±7)	p-side
3	191	16 (±7)	40 (±5)	p-side
4	195	14 (±8)	80 (±5)	p-side
5	573	19 (±6)	1269 (±28)	p-side

Measurements of alkaline phosphatase (PhoA) and β-galactosidase (LacZ) activities of sandwich fusions at the indicated positions within LTO1. Measurements were taken on at least two separate bacterial clones and correspond to an average of three representative measurements. The ±sd is indicated for those measurements. Fusion numbers correspond to those indicated in Figure 1. Values are expressed in arbitrary units for LacZ and Miller units for PhoA. The p-side corresponds to the bacterial periplasm and the lumen of the chloroplast, while the n-side corresponds to the bacterial cytoplasm and the stroma of the chloroplast.