Figure 4.
Arabidopsis Mutants with Reduced GluTRBP Contents.
(A) Immunoblot analysis of Arabidopsis Col-0 and two mutant lines (GluTRBP_antisense_#22, as22 and GluTRBP_sense_#34, s34) expressing reduced amounts of GluTRBP. Reduction in GluTRBP contents can be followed in etiolated, 48 h deetiolated, and 12-d-old plants. A detail of a Coomassie blue–stained 12% SDS-polyacrylamide gel served as loading control.
(B) Both mutant lines expressing reduced amounts of GluTRBP are hardly distinguishable from the wild type grown under standard growth conditions (90 μmol photons m−2 s−1). Since GluTRBP knockout is lethal in the very early stages of cotyledon development (see Supplemental Figure 10 online), the phenotypes of 48 h deetiolated plantlets is shown as an example. Magnification ×2.
(C) Protochlorophyllide content of 3-d-old etiolated Arabidopsis Col-0 and the two mutant lines GluTRBP_antisense_#22 and GluTRBP_sense_#34.
(D) Pigment content (Chl a, chlorophyll a; Chl b, chlorophyll b; and Car, carotenoid) of 48 h deetiolated Arabidopsis Col-0 and lines GluTRBP_antisense_#22 and GluTRBP_sense_#34. Seedlings were grown in darkness for 3 d before they were transferred to light.
(C) and (D) Given are means ± sd. t tests were performed, but differences between wild-type Col-0 and mutant lines were not significant (P > 0.05).