Table 1.
Protein Interactions between At-GluTR and At-GluTRBP Shown by Yeast Two-Hybrid Assays
| Gal4-AD |
|||||||
| LexA-BD | Empty Plasmid | At-GluTRBP | At-GluTRBP without TP | At-GluTR without TP (AtHEMA1) | At-GluTR without TP, without HBD (At-HEMA1) | At-GSAT | At-GluRS |
| Empty plasmid | − | − | − | − | − | − | − |
| At-GluTRBP | − | + | + | + | + | − | − |
| At-GluTRBP without TP | − | + | + | + | + | − | − |
| At-GluTR without TP (HEMA1) | − | + | + | − | − | − | − |
| At-GluTR without TP, without HBD (At-HEMA1) | − | + | + | − | − | − | − |
| At-GluTR (At-HEMA2) | − | + | + | − | − | − | − |
| At-GSAT | − | − | − | − | − | − | − |
| At-GluRS | − | − | − | − | − | − | − |
Protein interactions between At-GluTRBP and At-GluTR (encoded by either At-HEMA1 or At-HEMA2) were tested by different bait (columns) and prey (rows) protein combinations. Both proteins were cloned as full-length proteins as well as mature proteins without transit peptide (TP). Additionally, At-GluTR lacking its putative HBD also shows binding to At-GluTRBP. No protein interactions were detected using At-GSAT and At-GluRS as bait or prey proteins. Protein interactions are indicated by + and lack of interaction by −. Some of the data summarized here are shown in Supplemental Figures 2B and 6B online.