Table 2.
Lignin Content and Structure in the Wild Type and Transgenic Lines
| Line | Klason lignin KL (% DW) | AcBr Lignin (A280/gDW) | Alkali (A280/gDW) | Thioacidolysis Total Yield (μmol/g Klason Lignin) | S:G Thioacidolysis Molar Ratio |
| Wild type | 22.44 ± 0.40 | 91.61 ± 4.11 | 58.81 ± 2.02 | 1241 ± 119 | 1.03 ± 0.03 |
| SAD S4 | 22.90 ± 0.11 | 99.43 ± 1.84 | 66.31 ± 1.31 | 1222 ± 77 | 0.99 ± 0.02 |
| SAD S18 | 21.75 ± 0.46 | 88.94 ± 3.25 | 57.20 ± 2.36 | 1271 ± 56 | 1.09 ± 0.56 |
| SAD S21 | 21.92 ± 0.21 | ND | ND | 1287 ± 67 | 1.03 ± 0.02 |
| SAD S32 | 22.10 ± 0.25 | ND | ND | 1204 ± 58 | 1.02 ± 0.05 |
| SAD S45 | 22.23 ± 0.45 | ND | ND | 1315 ± 145 | 1.05 ± 0.04 |
| SAD CAD SC3 | 22.35 ± 0.18 | 100.93 ± 1.86 | 105.05 ± 4.44 | 975 ± 21 | 0.61 ± 0.03 |
| SAD CAD SC7 | 21.54 ± 0.45 | 108.99 ± 0.60 | 155.59 ± 13.36 | 641 ± 115 | 0.46 ± 0.08 |
| SAD CAD SC34 | 20.81 ± 0.52 | 85.04 ± 3.98 | 149.75 ± 17.03 | 875 ± 140 | 1.10 ± 0.09 |
Data are means and se corresponding to biological triplicates (three plants per line). Klason and acetyl bromide lignins were determined on EXR samples collected from the base of the stems of 10-week-old tobacco plants. Thioacidolyses are run from the same EXR samples, and the yields are expressed in μmoles per gram of Klason lignin. AcBR, acetyl bromide; DW, dry weight; gDW, g dry weight; ND, not determined.