Figure 3.

Lack of TAZ results in the accumulation of Nek1 protein but not RNA. (A) Cell lysates from wild-type or TAZ−/− mouse embryo fibroblasts were used for immnuoblotting. Western blots showed that TAZ−/− resulted in the accumulation of PC2 and Nek1. (B) Normal mouse kidney epithelial cells were infected with lentivirus targeting mouse TAZ and nontargeting lentivirus (Vector). Five days after infection, cells were prepared for western blot. (C) qRT-PCR for RNA levels of Aldolase, TAZ, Nek1, and Pkd2 in TAZ-depleted mouse kidney cells. TAZ depletion did not affect the RNA levels of Nek1 and Pkd2. *P < 0.001; **P < 0.0001. (D) Mouse embryo fibroblasts were infected with lentivirus targeting mouse TAZ and nontargeting lentivirus. Four days after infection, western blot was performed for PC2 and Nek1. (E) qRT-PCR for RNA levels of Aldolase, TAZ, Nek1, and Pkd2 in TAZ-depleted mouse embryo fibroblasts. *P < 0.001; **P < 0.01.