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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Curr Top Microbiol Immunol. 2010;342:291–308. doi: 10.1007/82_2010_27

Figure 4.

Figure 4

Creation of SVV mutants and recombinant viruses using the SVV cosmid-based recombination system. The relative genomic location of each 32–38 kb SVV fragment cloned into cosmid vectors is indicated. As an example, the arrow indicates the location for insertion of translational stop codons or a foreign gene into the unique KpnI site of ORF 14 (gC gene) within CosA. Following co-transfection of Vero cells, genetic recombination occurs resulting in the generation of infectious recombinant virus harboring the specified mutation or foreign gene.