Kv1 channel expression by IEC-Cdx2 cells. Immunblots of Kv1.5, Kv1.4 and Kv1.6 channel protein in total cell lysates (Lys), non-biotinylated (NB) and biotinylated (B) fractions obtained from IEC-Cdx2 cells subjected to cell surface biotinylation. After detection of Kv channel protein (upper panel of each set), each blot was stripped and reprobed with antibody against actin to assess loading (lower panel of each set). The apparent molecular masses of Kv1.5, Kv1.4 and Kv1.6 were ~75 kDa, ~96 kDa and ~56 kDa, respectively. The immunoblots shown are representative of n ≥ 3.