Co-association of Kv1.6 and Kv1.4 in IEC-Cdx2. Top: Immunoprecipitation (IP) was performed with antibody against Kv1.4, then the starting material (lane 1), IP (lane 2) and supernatant (lane 3) were immunoblotted (IB) directly with antibody against Kv1.6. Middle: IP was performed with antibody against Kv1.4, then the starting material (lane 1), IP (lane 2) and supernatant (lane 3) were IB with antibody against Kv1.6 pre-incubated with an excess of the fusion protein encoding the epitope used to generate the Kv1.6 antibody. Bottom: IP was performed with antibody against Kv10.1, then the starting material (lane 1), IP (lane 2) and supernatant (lane 3) were IB directly with antibody against Kv1.6. The immunoblots shown are representative of n ≥ 3.