Table 2.

Expression of ECM components (collagen I, collagen III, and fibronectin) and TGF-β1 from rat renal fibroblast cell line NRK-49F after 24 hours in culture

ECM Components	Control	TAM	IL-1β	Ang-II	IL-1β+TAM	Ang-II+TAM
Collagen I (ratio/GAPDH)	1.00±0.45	0.89±0.40	2.62±0.26a	2.83±0.22b	1.12±0.26c	0.94±0.53d
Collagen III (ratio/GAPDH)	1.00±0.80	0.60±0.50	1.75±0.17	2.72±0.17b	0.79±0.20	0.74±0.34d
Fibronectin (ratio/GAPDH)	1.00±0.69	1.02±0.33	3.08±0.20a	2.06±0.55	1.06±0.24c	0.98±0.49
TGF-β1 (ratio/GAPDH)	1.00±0.33	0.91±0.13	2.37±0.21a	2.16±0.21b	1.02±0.35c	0.51±0.21d

Control, nonstimulated renal fibroblasts; TAM, renal fibroblasts treated with tamoxifen; IL-1β, renal fibroblasts stimulated with IL-1β; Ang-II, renal fibroblasts stimulated with angiotensin-II; IL-1β+TAM, IL-1β–stimulated renal fibroblasts treated with tamoxifen; Ang-II+TAM, angiotensin-II–stimulated renal fibroblasts treated with tamoxifen; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

^aP<0.05 versus control and TAM groups.

^bP<0.05 versus control and TAM groups.

^cP<0.05 versus IL-1β (one-way ANOVA between control, TAM, IL-1β, and IL-1β+TAM groups).

^dP<0.05 versus Ang-II (one-way ANOVA between control, TAM, Ang-II, and Ang-II+TAM groups).