Figure 3.
Representative electron micrographs from Lias+/−Ins2Akita/+ mice at 7 months of age. (A) Segmental structural irregularities of thickened GBM, including seesawing of the lamina rara externa and lamina densa protrusions into Bowman’s space, vaguely resembling “humps” (white arrow). Loss of the fenestration in endothelial cells is also depicted (black arrow). Thinning of the lamina densa, sometimes located immediately adjacent to thickened segments with densa protrusions, was also observed (black arrow). (B) Structural irregularities of mitochondria, including dilation and disruption of cisternae and enlargement of lysosomes containing an electron-dense substance (presumed to be lipofuscins). (C) Scattered zebra bodies with laminated lipid particles in proximal tubular epithelium-like cells. (D) Mild podocyte effacement occurred in some areas of GBM (arrow). Magnification, ×8000 in A; ×5000 in B; ×20,000 in C; ×5000 in D.