Figure 1.

Renal cortical expression of TLR4, TLR2, and macrophage infiltration in human kidney biopsies. Representative photomicrographs of TLR4 staining in human renal cortical tissue from normal subjects (A), DN patients (B), and DM-NN patients (C); TLR2 staining in normal subjects (D), DN patients (E), and DM-NN patients (F); and CD68 staining, which denotes infiltrating CD68⁺ macrophages, in normal subjects (G), DN patients (H), and DM-NN patients (I). Negative control by omission of the corresponding primary antibodies demonstrated no nonspecific staining (J–L). Hematoxylin stain; original magnification, ×400.