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. 2011 Oct 11;23(1):86–102. doi: 10.1681/ASN.2010111210

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Copyright © 2012 by the American Society of Nephrology

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Figure 11. — Renal cortical CCL-2 expression and macrophage infiltration in diabetic and nondiabetic TLR4^−/− and wild-type mice with or without Unx. (A) Renal cortical CCL-2 mRNA expression determined by real-time PCR. (B) Representative photomicrograph of immunohistochemical staining for CCL-2. Hematoxylin stain; original magnification, ×400. (C) Quantitative analysis of tubular CCL-2 staining. (D) Representative renal cortical sections of F4/80 immunostaining. Hematoxylin stain; original magnification, ×400. (E) Number of interstitial F4/80⁺ cells. *P<0.05 versus the corresponding nondiabetic animals; ^#P<0.05 for comparison between diabetic Unx-TLR4^+/+ and Unx-TLR4^−/− mice.