Figure 4.

Effect of high ambient glucose on TLR4 and TLR2 mRNA expression in PTECs. Dose effect of HG on mRNA expression of TLR4 (A) and TLR2 (B). PTECs were incubated with increasing doses of ambient glucose (from 5.5 to 30 mM) for 8 hours. mRNA expression was determined by real-time PCR. *P<0.05, **P<0.01 versus PTECs cultured with normal glucose media. (C) Time effect of HG on mRNA expression of TLR4. PTECs were incubated with high ambient glucose (30 mM) for 0–24 hours. ^†P<0.05, ^††P<0.01 versus time zero control. All results represent means ± SD obtained from five independent experiments. NG, normal glucose.